Determination of Acarbose in Tablets by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy

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Attenuated total reflectance Fourier transform infrared (ATR- FTIR) spectroscopy method was developed for the quantitative determination of acarbose in tablets. Two calibration curves were generated. The first calibration curve was based on peak height at 2928.54 cm-1 with correlation coefficient of 0.9987. The second calibration curve was based on peak area which range from 2953.39-2851.04 cm-1 with correlation coefficient of 0.9978. The FTIR method was validated according to International Conference on Harmonization (ICH) guidelines. The method was precise over a range of 40%-100% w/w with % RSD values less than 2 for intra-day and interday precision. The accuracy of the method was expressed as the percentage recovery of the spiked acarbose standards with a mean recovery of 106.9%. The limit of detection and limit of quantification values were 0.004% and 0.012 % w/w, respectively. The validated method was used for the quantification of acarbose in tablets with good results.

The quantitative determination of acarbose in tablets by the FTIR method was carried out without the extraction of the active pharmaceutical ingredients. It only required finely grinding the tablet and applying the powder on the ATR-FTIR instrument. The results obtained for the quantification of acarbose in tablets by FTIR method in the present study is comparable to work reported by Cherkaoui et al. [6]. In their work, they used high performance liquid chromatography equipped with evaporative light scattering detector (ELSD) for the analysis of acarbose in tablets without any derivatization procedure. Although their method is sensitive, however, it is expensive and requires more training to operate the ELSD due to its dependency on the usage of the melting point properties of the substance in which the temperature to be used in the nebulizer should be very low in comparison to the melting point of the sample being analyzed so that only the mobile phase can evaporate leaving the analyte without evaporating.  In comparison, ATR-FTIR is faster, simpler and cost efficient.

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Nicola B
Editorial Manager
Journal of Biochemistry & Biotechnology
Email: biochembiotech@scholarlypub.com